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課程名稱 |
幹細胞與組織工程應用
APPLICATIONS OF STEM CELL AND TISSUE ENGINEERING |
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開課學期 |
97-1 |
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授課對象 |
學程 幹細胞與再生醫學學程 |
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授課教師 |
林峰輝 |
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課號 |
Prog5148 |
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課程識別碼 |
P30 U3040 |
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班次 |
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學分 |
1 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
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上課地點 |
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備註 |
初選不開放。暑期課程，請於6月18日前洽生技中心報名。
總人數上限：25人 |
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課程簡介影片 |
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核心能力關聯 |
本課程尚未建立核心能力關連 |
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課程大綱
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為確保您我的權利,請尊重智慧財產權及不得非法影印
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課程概述 |
本課程將配合胚胎幹細胞與成體幹細胞特性之不同，分別將以生物性分解材料及高分子材料等設計，檢驗幹細胞的組織功能。 |
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課程目標 |
Introduction to tissue engineering, Stem Cell (Ⅰ): introduction to stem cell, stem cell harvest & isolation
Stem cell (Ⅱ): stem cell observation, stem cell proliferation & identification
Itrodution to cell culture: primary culture and cell line, cell counting, staining, re-plate, expansion, frozen
Introduction of cyto-toxicity: MTT & LDH test, practice
Introduction to geno-toxicity: chromosome aberration, sister chromatid exchange, abservation of chromosome, practice
Itroduction to collagen: to isolation type Ⅰ collagen, to prepare collagen membrane, to practice
Introduction to fibrin glue: preparation of fibrinogen & thrombin, how to prepare fibrin glue, practice
Artificial extra cellular matrix (Tri-copolymer, HA-C6S- Gelatin) as scaffold for artificial cartilage tissue engineering
Material on immuno-isolation: microsphere preparatiton for bio-artificial parathyroid and pancreas
Introduction of animal study
Practice: subcutaneous implantation & practice |
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課程要求 |
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
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參考書目 |
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評量方式
(僅供參考) |
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